LOCUS       BG077867                 554 bp    mRNA    linear   EST 12-MAY-2010
DEFINITION  H3021H12-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
            H3021H12 5', mRNA sequence.
ACCESSION   BG077867
VERSION     BG077867.2
DBLINK      BioSample: SAMN00163148
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
            Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
            Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
  TITLE     Genome-wide expression profiling of mid-gestation placenta and
            embryo using a 15,000 mouse developmental cDNA microarray
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
   PUBMED   10922068
COMMENT     On Jan 26, 2001 this sequence version replaced BG077867.1.
            Other_ESTs: H3021H12-3
            Contact: George J. Kargul
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            Email: cdna@lgsun.grc.nia.nih.gov
            This clone set has been freely distributed to the community. Please
            visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
            Plate: H3021  row: H  column: 12
            Seq primer: -21M13 Reverse
            POLYA=No.
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="niaEST:H3021H12-5"
                     /db_xref="taxon:10090"
                     /clone="H3021H12"
                     /sex="clones arrayed from a variety of cdna libraries"
                     /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
                     /dev_stage="Clones arrayed from a variety of cDNA
                     libraries"
                     /lab_host="DH10B"
                     /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
                     clone is among a rearrayed set of 15,247 clones from 11
                     embryo cDNA libraries (including preimplantation stage
                     embryos from unfertilized egg to blastocyst, embryonic
                     part of E7.5 embryos, extraembryonic part of E7.5 embryos,
                     and E12.5 female mesonephros/gonad) and one newborn ovary
                     cDNA library. Average insert size 1.5 kb. All source
                     libraries are cloned unidirectionally with Oligo(dT)-Not
                     primers. References include: (1) Genome-wide expression
                     profiling of mid-gestation placenta and embryo using a
                     15,000 mouse developmental cDNA microarray, 2000, Proc.
                     Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
                     cDNA analysis reveals phased gene expression patterns
                     during preimplantation mouse develolpment, 2000,
                     Development, 127: 1737-1749; (3) Genome-wide mapping of
                     unselected transcripts from extraembryonic tissue of
                     7.5-day mouse embryos reveals enrichment in the t-complex
                     and under-representation on the X chromosome, 1998, Hum
                     Mol Genet 7: 1967-1978."
BASE COUNT          171 a          130 c          117 g          136 t
ORIGIN      
        1 ctgcagagaa atcttgcgga tcacagaccc aaaaatgaca tgttacatag accagctgaa
       61 cacttggtat gacgtgaaaa ctcatcaaga agtgaccagc agtcgcctct tctcagaaat
      121 ccagaccacc ttggggactt ttggtctgaa tggattggac aggcttctgt gcttcatgat
      181 tgtgaaagag ctacagaatt tcctcagtat gtttcagaaa attatcctga aagagagagc
      241 tgttcaagaa actttaaaga tgctcatgag tgctgttaac cctctaaaga gcatagtggc
      301 aaattcaagt aaagtttatc tttctgccat caccaaaaca cagaagatct ggagtgctta
      361 cctggaggct ataatgaagg ttggacagat gcagatcctg agacaacaga tcgccaatga
      421 gctaaactct tcctgtcgat ttgattccag acatctggca gctgccctgg ataatctcaa
      481 taaggctctc ctggcagaca tcgaagccca ctaccgggac ccttcgcttc cttaccccaa
      541 agaagataac acac
//