LOCUS BG077732 590 bp mRNA linear EST 12-MAY-2010 DEFINITION H3019A12-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3019A12 5', mRNA sequence. ACCESSION BG077732 VERSION BG077732.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 590) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG077732.1. Other_ESTs: H3019A12-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3019 row: A column: 12 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..590 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3019A12-5" /db_xref="taxon:10090" /clone="H3019A12" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 140 a 174 c 170 g 106 t ORIGIN 1 tttccgcata caaccagtga gtggagcctg cccgcgcaga gccgtctcga gcatagcccg 61 gagcctgaat cgagcagaac cagcctgaac tagcccagtc cggtgatgga gcgtccacag 121 cccgacagca tgccccagga tttgtctgag gccttgaagg aggccaccaa ggaggtacac 181 atccaagccg agaatgctga gttcatgaag aactttcaga agggtcaggt gtccagagaa 241 ggctttaagc tggtgatggc ttccttgtac catatctaca cggccctgga agaggagata 301 gagcgcaaca agcagaaccc agtctatgcc ccactctact tccctgagga gctgcaccga 361 agggctgccc tggagcagga catggccttc tggtatgggc ctcactggca ggaaatcatc 421 ccttgcacgc cagccacaca gcactatgta aagcgtctcc acgaggtggg gcgcactcac 481 cctgagctgc tggtggccca cgcatatacc cgctacctgg gtgacctctc agggggtcag 541 gtcctgaaga agattgcaca gaaggccatg gccttgccca gctctgggga //