LOCUS BG077728 700 bp mRNA linear EST 12-MAY-2010 DEFINITION H3019A08-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3019A08 5', mRNA sequence. ACCESSION BG077728 VERSION BG077728.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 700) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG077728.1. Other_ESTs: H3019A08-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3019 row: A column: 08 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..700 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3019A08-5" /db_xref="taxon:10090" /clone="H3019A08" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 154 a 190 c 214 g 142 t ORIGIN 1 tgttattttg agcaggagaa tgactggtgg gtgtgcaaac acataaaaaa gcccatccgc 61 tccacagtgc tcagcctgga ctggcatccc aacaacgtgc tcctggcagc aggctcctgt 121 gacttcaagt gcaggatctt ctctgcctat atcaaggagg tggaagaacg gccagcccct 181 acaccgtggg gctccaagat gccctttggg gagctgatgt ttgaatcgag cagcagctgt 241 ggctgggtgc atggggtctg cttctcggcc agtgggagcc gagttgcttg ggtcagccat 301 gacagcactg tatgcctggt agatgctgac aagaagatgg ccgtggcaac cctggcctct 361 gagacattac cactcctggc cgtcaccttc atcacagaaa atagtctagt ggcagcgggc 421 cacgactgct tcccggtgct gtttacctat gacaatgctg cggtgacatt gagcttcggt 481 ggccggctgg atgtacccaa gcagagctcc cagcgtggca tgacagcccg agagcgcttc 541 cagaacctgg acaagaaggc cagctctgag gggggtgcag ccactggggc cggcctggat 601 tcactgcaca agaacagtgt cagccaaatc tcggtgctca gcgggggcaa ggccaagtgc 661 tcacagttct gcactacagg catggatggc ggcatgagca //