LOCUS BG077710 614 bp mRNA linear EST 12-MAY-2010
DEFINITION H3018G08-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3018G08 5', mRNA sequence.
ACCESSION BG077710
VERSION BG077710.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 614)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG077710.1.
Other_ESTs: H3018G08-3
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3018 row: G column: 08
Seq primer: -21M13 Reverse
POLYA=No.
FEATURES Location/Qualifiers
source 1..614
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3018G08-5"
/db_xref="taxon:10090"
/clone="H3018G08"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 132 a 179 c 183 g 120 t
ORIGIN
1 gtcagcgctg gagcttccgc aggcgccatg gaaaaggtgc tggtcacagg tggggctggc
61 tacatcggca gccacacggt attggagctg ctggaggcag gctactcacc tgtggtcatt
121 gacaacttcc ataacgccat tcgtggagaa gactccatgc ctgagagcct gcgccgggtc
181 caggagttga caggccgctc tgtggagttt gaggagatgg acatcttgga ccaggcagcc
241 ctacagcacc tctttaagaa gcacagcttt aaggccgtca tccactttgc tggcctcaag
301 gctgtgggcg agtcagtgca gaagcctctg gactactata gagttaactt aacagggacc
361 atccagcttc tagagatcat gagggcccac ggagtgaaga acctggtgtt cagcagctca
421 gccaccgtgt acgggaaccc ccagtacctg cctctggatg aggcccaccc cacggggggc
481 tgtaccaacc cctacggcaa gtccaagttc ttcatcgagg agatgatccg ggacctgtgc
541 cgggcagaca cggcctggaa cgccgtgctg cttcggtact tcaatcccat aggcgcccac
601 gcctctgggc gcat
//