LOCUS BG077709 621 bp mRNA linear EST 12-MAY-2010 DEFINITION H3018G07-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3018G07 5', mRNA sequence. ACCESSION BG077709 VERSION BG077709.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 621) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG077709.1. Other_ESTs: H3018G07-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3018 row: G column: 07 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..621 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3018G07-5" /db_xref="taxon:10090" /clone="H3018G07" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 175 a 149 c 141 g 156 t ORIGIN 1 cggagttggg aacacgcata ggtgttggga cttgtaggcc agcccatggc ataagccacc 61 taagaagtct acatctgaag acccttagtg ggtctgcaag gcccctgctc ctgatgggct 121 cccatcctgc tgtttacacg agtctgaact ttaagacaac agatgagcac tttatacttg 181 cctgcgtctt tccggggtca ctaggacagc ctgagtgtcc tctgagctgg cactgcagcc 241 cctccctcat ggatacatga gctagctgat cactaactct tatgtatgct taattctcac 301 attatcagac ctctgagcca ggtggtaggc tcaggggaat ttaaataaat aaataaagta 361 gttggtatac catcaaaagc ctgcaaggaa ggtgtgggat tcctgagagc caggaacttg 421 ctgagttcca gtgtgccatt tccccgtgtc agtcaggaca ctgggattac aagcatggac 481 tactgtaccc aggggtgatg aggtttcacc tttccattct aaatcaagtt gccttgtctc 541 tctggttact aagaactaca tacattacgg aatccaacaa gcacagaaca caattaaaaa 601 taaaaaaaaa atccagaaaa c //