LOCUS BG077640 597 bp mRNA linear EST 12-MAY-2010 DEFINITION H3017H03-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3017H03 5', mRNA sequence. ACCESSION BG077640 VERSION BG077640.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 597) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG077640.1. Other_ESTs: H3017H03-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3017 row: H column: 03 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..597 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3017H03-5" /db_xref="taxon:10090" /clone="H3017H03" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 158 a 136 c 149 g 154 t ORIGIN 1 ctctggttct gggaatagac tggatgggaa gaaaaaaggg gttgagccca gtccctcccc 61 aatcaagcct ggagacatca aaagaggaat tcctaattac gaatttaagc ttggtaagat 121 cactttcatc agaaattcac gtccattggt caaaaaggtt gaagaggatg aagctggagg 181 cagattcatt gctttctctg gagaaggaca gtcactgcgt aagaagggaa gaaagcccta 241 agtctaaaac tgttggctga ctggaaaata aaagatccga ttgcacctgg cttacagtta 301 ctggctctga caagttcaga gatactctgt tatttatggt ttgtttaaag ttacctgaga 361 actatcaagc ttgtcctgga tacaaagcag cagaacttgg aggttgtgtg cttgatctgg 421 gggagagagc gctggggtcc tctaggtggt ggcctcctcc actccctaac tccagcttca 481 gggccctcac agcctcagca gagcagctcc cgattcctga ctcactcttc ttccccatgt 541 ctgttcatgg tgcagtgtct ttagcccatc atggctactc aggccagcac ttgagta //