LOCUS BG077455 587 bp mRNA linear EST 12-MAY-2010 DEFINITION H3015E11-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3015E11 5', mRNA sequence. ACCESSION BG077455 VERSION BG077455.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 587) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG077455.1. Other_ESTs: H3015E11-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3015 row: E column: 11 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..587 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3015E11-5" /db_xref="taxon:10090" /clone="H3015E11" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 149 a 175 c 147 g 116 t ORIGIN 1 gggagccccg cgcgccgtgc agttctgtgc cttgcctgtc tgtcccttcc gattgtgcct 61 agcgatgacc acccagcaga ttgttctcca gggcccgggt ccctggggtt tccgcctcgt 121 gggcggcaag gacttcgaac aacctctcgc catttcccgg gtcactcccg gaagcaaggc 181 tgctatagcg aatttatgta ttggagattt aatcacagcc atcgatgggg aagataccag 241 cagcatgaca cacttggaag ctcagaacaa aatcaagggc tgcgcagaca acatgacgct 301 cacagtatcc aggtctgaac agaagatttg gtctcctcta gtgaccgagg aggggaaacg 361 tcatccctac aagatgaatt tagcgtcgga accccaagaa gtcctgcaca taggaagtgc 421 ccacaaccga agtgccatgc catttactgc ctcacctgct cccagcaccc gggtcatcac 481 aaaccagtac aacagcccaa ctggcctcta ctcatccgaa aacatctcta acttcaacaa 541 tgctgtggag tcgaagacct cagccagtgg ggaggaggcg aacagca //