LOCUS BG077441 598 bp mRNA linear EST 12-MAY-2010 DEFINITION H3015D08-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3015D08 5', mRNA sequence. ACCESSION BG077441 VERSION BG077441.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 598) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG077441.1. Other_ESTs: H3015D08-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3015 row: D column: 08 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..598 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3015D08-5" /db_xref="taxon:10090" /clone="H3015D08" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 127 a 185 c 166 g 120 t ORIGIN 1 ggccgattgc gtcttcccag cccaagcctt ccagcacccg gtgccagggg ccatggagcc 61 ccgggcagtt gcggatgcct tggagaccgg agaggaagat gcggtgacag aagctctgcg 121 gtcgttcaac cgggagcatt ctcagagctt caccttcgat gatgcccagc aggaggacag 181 gaagagactc gcaaagctac tggtctccgt cctggagcag ggcttgtcac caaagcaccg 241 tgtcacctgg ctgcagacta tccgaatcct atcccgagac cgcagctgcc tggactcatt 301 tgccagccgc cagagcttac atgcactagc ctgctatgct gacattaccg tctcagagga 361 acccatccca cagtccccag acatggatgt cctcctcgag tctctcaaat gcctgtgtaa 421 tcttgtgctc agcagtccaa cagcacagat gctagcagca gaggctcgcc tggtggtgag 481 gctagcggag cgtgtgggac tgtaccgcaa gaggagctat ccccacgaag tccagttctt 541 tgacttgagg ctccttttcc tgctaacagc ccttcgcacg gatgtgcgcc agcaactg //