LOCUS BG077392 711 bp mRNA linear EST 12-MAY-2010 DEFINITION H3014H04-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3014H04 5', mRNA sequence. ACCESSION BG077392 VERSION BG077392.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 711) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG077392.1. Other_ESTs: H3014H04-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3014 row: H column: 04 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..711 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3014H04-5" /db_xref="taxon:10090" /clone="H3014H04" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 138 a 193 c 184 g 196 t ORIGIN 1 gccctttcca taaacacgcc cggttgcatg cagagtgtgc agtttatgtc ttctggagaa 61 ctcatggtct cgtgggttcc caagacgggc agcgccccaa acttgcttaa cttgtcttca 121 gaggcccagc agccactttg tcgcccactt gagctggtgc cgtggctgcg gaaggatgac 181 gcccctctgc acagtcccag catgcccttt ctcggagccc ctccccatgc tagtgtgcca 241 gcacccccta accgagaccg cgcaaggccg cagcagagga agcttggcca ccagagtgga 301 cagaggggcc ggagaggctt cgcccggggg tttgtctcct tgtccgcagt gagcgtgtct 361 gtgtcccgct tgtactgttt ggaaatgacc tactggaatt gcaaaaccta gtttctcttt 421 acatttcagc cttgaccttt gctgtgttct agaacagtca tgtgagggta gaactgaaga 481 gggagagcgg gagacctgcc gtgagcttgg ttccttctcc ttcctttact gttgagaagt 541 gtgcctgcct ctgatgctgt ccccagtgcc gcgcggctgc tcctccatcc tattgataca 601 gtattgtgca tgctggtgtt gtatttctat acggtagctt gacatttatt accttacact 661 gtaggtgtta tgtatcctat gacaaaaaaa cttaagactt caattttttt t //