LOCUS BG077325 628 bp mRNA linear EST 12-MAY-2010 DEFINITION H3014A07-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3014A07 5', mRNA sequence. ACCESSION BG077325 VERSION BG077325.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 628) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG077325.1. Other_ESTs: H3014A07-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3014 row: A column: 07 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..628 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3014A07-5" /db_xref="taxon:10090" /clone="H3014A07" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 129 a 203 c 176 g 120 t ORIGIN 1 gagttcctca ttgtgcacta tgagcagatc tttggaatgg atgagctccc tctggcctcg 61 gagcctctga cccaagaccc tggcttggct cccgcatgcc ttgaatccag tccccagcac 121 ccagcctcac ttcttgccca agacacacag cccctgacca tagccttgga ttccagccca 181 gaccccaaac accacagtgc cttggagaag tgccccgagg tcacacctcc tgagcttgca 241 actctgcaga gggaccagag ggaggaggag gtggaagaca ccagagatgg ggcaggggat 301 gggtccagcc actgccctga ggacttggcc ctgggagcac aatcccgggg ccacttcagc 361 cgccagccag tgaagtattc ccggggaggt gtgaggccag ttactcatca actctccagc 421 ctggctctgg tggcctccaa gctgtgtgag gagactcctg tcactgtctc agcagtgcac 481 cgaggtagtt tgagggtacg aggtttgggc cctgctgctg cctgccctga aggcagcccc 541 ctgcgccgga accctctgcc caagcacttt gagatcaccc aggagacagc ccgacttctc 601 tccaagctga acagtgatgc tgtgtcca //