LOCUS BG077130 663 bp mRNA linear EST 12-MAY-2010 DEFINITION H3011B09-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3011B09 5', mRNA sequence. ACCESSION BG077130 VERSION BG077130.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 663) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG077130.1. Other_ESTs: H3011B09-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3011 row: B column: 09 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..663 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3011B09-5" /db_xref="taxon:10090" /clone="H3011B09" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 182 a 151 c 179 g 151 t ORIGIN 1 cctttccggc cctgtttcca gacgcctggg gtcgtgattg ctagggtgga tcttcggttt 61 gctcagaagc cgctgtggtc gagaatgagc ggaaccctgg gaaaggtgct gggtgtgtgg 121 accaatactg tttctaagca aggcttttcc ctcctaaggt tcagaagttt gggagagaat 181 cccatttttt ctgcaggtgg tattctgtgg accagtcggc actacaagac aaagcccact 241 cgtggcatcg gaagatacag acacttggtg aaagtgcagg agcccaaaaa gaagaaagca 301 aaagtagaac tgagagccat taatgtgggg acagattatg aatatggggt tttaaacatc 361 cacctgacag catatgacat gagtctggca gaaagttatg cccagtatgt ccaccgcctc 421 tgcaaccggc tgtccatcaa agtcgaggaa agctatgcga tgcctaccaa aaccatggaa 481 gtgatgcgac tgccagacca aggcaacaaa atggtcctag actccatcct taccacccat 541 gagcgagtgg tgcagatcag cggtctgagt gctacatttg cagagatttt cttggaagta 601 ctccaaagca atcttccaga aggagtcaga ttgtccgtga gggagcacac tgaagaagac 661 ttc //