LOCUS BG076954 595 bp mRNA linear EST 12-MAY-2010 DEFINITION H3009B03-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3009B03 5', mRNA sequence. ACCESSION BG076954 VERSION BG076954.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 595) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG076954.1. Other_ESTs: H3009B03-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3009 row: B column: 03 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..595 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3009B03-5" /db_xref="taxon:10090" /clone="H3009B03" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 156 a 148 c 140 g 151 t ORIGIN 1 ggcttcggta gcgaccggct gtcttctgct gcttgagcgg cgcccgcacc ttccctagga 61 gctcgcagca gccggctggc ctctgctcca cggtaaccat gtgcgaccgg aaggcggtga 121 tcaaaaatgc agacatgtcg gaagagatgc aacaggactc ggtggagtgc gctacccagg 181 cgttggagaa gtacaacatc gagaaggata ttgcggccca tatcaagaag gagtttgaca 241 agaagtacaa ccctacctgg cactgcattg tgggccgaaa cttcggtagt tatgtgacac 301 atgaaaccaa acacttcatc tacttctacc tgggtcaggt ggccattctt ctgttcaaat 361 ctggttaaaa gcatggactg tgccaaacac ccagtgatcc atccaaaaac aaggactgca 421 tccaaattcc aaataccaga gactgaatct tcagccttgc taagggaaca cctcgtttga 481 atctgttgtg ttgtgtacag ggcttcattc tctgtacaag tctgtggtta taaaattagt 541 aaaacagctt acatttgtat ttattttcta gtccatactt ctgtaccccc atttt //