LOCUS BG076607 596 bp mRNA linear EST 26-JAN-2001 DEFINITION H3003E08-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3003E08 5', mRNA sequence. ACCESSION BG076607 VERSION BG076607.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 596) AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A., Tanaka,T.S., Carter,M.G. and Ko,M.S.H. TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set JOURNAL Unpublished COMMENT Other_ESTs: H3003E08-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3003 row: E column: 08 Seq primer: -21M13 Reverse POLYA=Yes. FEATURES Location/Qualifiers source 1..596 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3003E08-5" /db_xref="taxon:10090" /clone="H3003E08" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 181 a 142 c 130 g 143 t ORIGIN 1 gtgcggcggg cgcccttttt tttttttttt ggtttttcga gacagggttt ctctgtatag 61 ccctggctgt cctggaactc actttgtaga ccaggctggc ctcgaactca gaaatccgcc 121 tgcctctgcc ttccaagtgc tgggattaaa ggcgtgcgcc accacgcctg gctccacatc 181 ccaagtttta agaggggctt ctacactaat aactaggtgt gatgaaatat aaagcaaaat 241 atgaagtatt catacaataa atcacagtgg caaagtcttg aacctctaga aacagcctac 301 actgattggc aataaagaga aaataaagaa aatcaagaac acccagcatg agctggtttc 361 aagtcactgg gacatctgaa accccaattg ccaaggcaga gatgcagaaa gccttaaaat 421 actaaacagc caggtgggga ctagagagat ggctctgcac ccatatggtg gtttacaacc 481 atctggaact cccgttccag gggagctaat acctttttct agcctccaaa gacaccaggc 541 atgcaagttg ccctcagaca tacatgcagg caaagtacgc attaaaaaaa aattta //