LOCUS BG076368 690 bp mRNA linear EST 12-MAY-2010 DEFINITION H3158H07-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3158H07 3', mRNA sequence. ACCESSION BG076368 VERSION BG076368.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 690) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG076368.1. Other_ESTs: H3158H07-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3158 row: H column: 07 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..690 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3158H07-3" /db_xref="taxon:10090" /clone="H3158H07" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 161 a 137 c 198 g 194 t ORIGIN 1 agttttttcg aaacagggtt tctctgtata accctggctg tcctggaact cactttgcca 61 cagccgagca agaagatgct ggaagtgtgt aggagttagc cagggggcca gacatgtgtg 121 cccaggttgt acacagggct cacggagact gcaaggcaat gggtaatacc attcctgtgc 181 tgcaggtggg agctaagact ggcacaagga catcctttag ctggcttagt acagtgtggc 241 tgggtagact cagcaatacc aatctgggac agcctagctc agaggaatcc tgcctgcctg 301 atgccatgcg ttcttgcagt gatggtgatg atggcgttgg tgttggtgat gatggtgata 361 gtagtggtgt tggtgatgat ggtgatggta gtggtgatgg tgatgatgat ggactttcac 421 ttttttgaaa ctgtaagtcc ttttgaaagt tgccttggtc atgttgttat cacagcaata 481 ggaaaacaac taatagaggg ggcctccagg accctctcta tgaccagttc tcttccagag 541 ccttggaaca acacacccct ccagtcccat tactcccatt aggttttgta ctcccagtca 601 aagtcttact cattgtaatg ggttgaatga gaatggtttg aatacttggt ccccagttgg 661 tggaactgtt tgggaaggat taggaggtgt //