LOCUS BG076344 712 bp mRNA linear EST 12-MAY-2010 DEFINITION H3158F04-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3158F04 3', mRNA sequence. ACCESSION BG076344 VERSION BG076344.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 712) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG076344.1. Other_ESTs: H3158F04-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3158 row: F column: 04 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..712 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3158F04-3" /db_xref="taxon:10090" /clone="H3158F04" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 226 a 147 c 150 g 189 t ORIGIN 1 cctctctctc tcaaagaaag aatttcaccg acactatctc tggtgaatgt ggacccagca 61 tcagccgatt ttcttaaaga ggccagaaat gacagagcaa gtcaaaacaa acaagaaaaa 121 acattttttt ttgggcgggg ttagatgggt ggcatgcaca ttcctgtaat tccagcactt 181 gcaaggctga gacaggaaga tcatgacttc aaggccagcc tgacttaaac aaaacaacaa 241 aaaatacccc ccccaataat aaacaacaaa acccaaaatt tgtccatgaa ccccagggaa 301 ctgtggtaga aaatgaatac taaaggcatt aggtgataat taactgtcaa tgtatattca 361 ctgatgggac cactgggcta ccatataggc tgtgtctctt tcctgtcaca agagaacata 421 attctacagt ggggcagtct gagtgattgc tccctggaga aactcagttc catttaattt 481 catactcacc cacttgtgtc tttaagatta gaaaggagat gatcagaaaa aggggggtgt 541 ataaatctct ggaattgtgt accccagtgt atcagtaaat cacgttttgg atttcctact 601 ggattcctga gtacctcagc atttctctaa tctggagagt agtggcagct ctgggttacc 661 tgacaaatta tgattctagt gttgaaagca gactgtgagc ttggtcaata gt //