LOCUS BG076294 803 bp mRNA linear EST 12-MAY-2010 DEFINITION H3158A06-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3158A06 3', mRNA sequence. ACCESSION BG076294 VERSION BG076294.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 803) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG076294.1. Other_ESTs: H3158A06-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3158 row: A column: 06 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..803 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3158A06-3" /db_xref="taxon:10090" /clone="H3158A06" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 204 a 220 c 199 g 180 t ORIGIN 1 cactggtaaa aacagtttat taagagagaa agcactccca ggtttcgaag tgggccagcg 61 gtggagcaaa gccaagggcc cagctgccct gaaccttcag cattaagacc cttacaggtc 121 atctacagag ccagcaggct tctttggcat gtactacctg tccctctaag ggcccttcta 181 gacttttttt gcttaggttg ctttacatat gctaatcttg gggcctttcc tggctcgttc 241 tcttatggca ggcgtaaggt caccccaagg caccaaggtg gccctgccta ggtaagctac 301 agggcctccc ccaattaatg gctctaacga agacaacttt accaatggca tctggcgcct 361 cagaatgacg agcaacagtc actcaggtga catccctctc ctgaagaaga aagaaaaagg 421 acagtgtcct gaccagcctt gtcctgaaaa aacaaaaaat tccattctcc caggctatgt 481 ctaaggacag ctgggacaca gtgccccgta aagatattct aggcatctcc aagggtaggc 541 ttgatacagt gatactaggg ctccccctag cggctgagaa acaacatgaa cctcaacgtc 601 aaggccccga gatgcttcct gtcccagacc ttccagctca aacagaaagt gctttattgg 661 agggtatggc tccttcaggc ctcccttgag gaagggtcag acagtgccca gagctttggg 721 cctcagcagg taagtcccag ggaccatgct ggtgggcagc atttctgcca gccctcagca 781 caggagaggg gtctgctttg ctg //