LOCUS BG076281 568 bp mRNA linear EST 12-MAY-2010
DEFINITION H3157G11-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3157G11 3', mRNA sequence.
ACCESSION BG076281
VERSION BG076281.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 568)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG076281.1.
Other_ESTs: H3157G11-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3157 row: G column: 11
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..568
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3157G11-3"
/db_xref="taxon:10090"
/clone="H3157G11"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 208 a 127 c 107 g 126 t
ORIGIN
1 ctgaaatctg gattttatat taaaaaatgt tacatatatt tttgacaaaa aaattcttag
61 acgtatgagg atagcagtgg aaacacagcg tacgcaccta gtacaattat gtaagaggca
121 aaatataaac ttacacgaac gcaaaacgag aacaaacctt gcctacagta gcctctagcg
181 agggccgggt atctttgccg gtgtgaaaca gccgcaacag acaaacgatg acacagaaaa
241 acaaacaatc tcctgaaaaa caaagcagca gcgacagccc aaagcgcctg ccacccgggt
301 tgtcctcaac tcttgtttgt gtcacttttt aaaagaacac aatttttctt gaaaggctcg
361 ctctcttagc tagctagctg ctgaactacg gtgtaaaaga aacaatgcaa gtccgtgtta
421 aaagaaaaac aaaaaacaaa aaaatttatt tgggaattga caagggacgc cctcacacac
481 gtacaaacac aacaaaagtg ctctagttcc ctctagaaaa tttggtcccc gaaagacatt
541 actcaaccac caggtcacgg gatttcgg
//