LOCUS BG076120 592 bp mRNA linear EST 12-MAY-2010 DEFINITION H3155H06-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3155H06 3', mRNA sequence. ACCESSION BG076120 VERSION BG076120.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 592) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG076120.1. Other_ESTs: H3155H06-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3155 row: H column: 06 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..592 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3155H06-3" /db_xref="taxon:10090" /clone="H3155H06" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 161 a 147 c 148 g 136 t ORIGIN 1 tttttaaaat gtaaagttgt gagtttattg catatgtaac aaaatgaacc tgaccttccg 61 ggtccagcct gctctacagt caacgcttcg tgtttccagc tggttccaca actgcattaa 121 atagaactag cgtttcttta aatactttcg attctgacat aaaacagaac attagtgtac 181 aactgtcaca ggaaaatgga caataaaaac tgggagagca actagggcta gcagcagcac 241 ttcaaacagg accttacaaa ataacttaga atacattata aacacagttt aaaaatccac 301 accgtcagaa ggctgccatg tggcccacag gctgcagtag ggtggggcgt gttctctgca 361 gaaggtcagt ccagcgccca cctgacctga acctggccat gtggcaagcc agtgcagggt 421 cagtaatcca ccttgtggca gatcctccta accaagcatg agactgcgac ctgctgccac 481 agcactgctg cagcctggcg cgtcctctct gaagcactgc ggagtagagt ggtgtctgct 541 gcgtccctga gtgtgggtgc cggccgctgg tgaggagagc ctgcggagca gg //