LOCUS BG076087 609 bp mRNA linear EST 12-MAY-2010 DEFINITION H3155E03-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3155E03 3', mRNA sequence. ACCESSION BG076087 VERSION BG076087.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 609) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG076087.1. Other_ESTs: H3155E03-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3155 row: E column: 03 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..609 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3155E03-3" /db_xref="taxon:10090" /clone="H3155E03" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 138 a 159 c 162 g 150 t ORIGIN 1 atcagattaa cgtttattag ttcaacaatc ccaccttccc atgcctcaca gagcgtccgt 61 gctcctcagt actgctgtgg ggatacaagg gcgccgggga agtgaagagg cccacgactc 121 caataacaca gcccatgtct ctggaagaag ccagatggct atatcatcca gtttgatctt 181 gtcaggcaac tgaagtagcc tctaaggaat gggtgcagcc tcagtctaag ggtgaggccc 241 aggtgtttta tttatttatc cagtcaggaa ggatgttcca gtcttggccc ctggtcatct 301 tgattaggga gccaagggaa ggagtcctgg gactgttctg gggaagtcaa gctggggggt 361 ccttgcaaca tcgaaggctc atattccgag tcagagtcta gctcaatccc agtgctctgg 421 aagtcagcat acgtttcctt ggggatggct ggcagttcct cctcatactc ggtctgatag 481 tcagactcat catccactgc agccaccttg gccggctcaa tccgtgacac aatctcagca 541 aggtccgtga aggaggaatt ggggcaggtg aacacccaca gtccttcctc ttgcttgtcc 601 cctgctggt //