LOCUS BG076076 668 bp mRNA linear EST 12-MAY-2010 DEFINITION H3155D04-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3155D04 3', mRNA sequence. ACCESSION BG076076 VERSION BG076076.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 668) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG076076.1. Other_ESTs: H3155D04-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3155 row: D column: 04 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..668 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3155D04-3" /db_xref="taxon:10090" /clone="H3155D04" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 179 a 163 c 150 g 176 t ORIGIN 1 ccttagtaca caagtttgct ttatttagta aagggtaaat tttatatgta tcccaaaatt 61 gtttaagaag tatattttac tattatcagt aaatattgtt gtgtatacct atgtatatac 121 taaagccaga gttgcaacaa tttcttggga aaaggagtca agtgggaaac tcgagagtag 181 tcctgtggta gagcttcatg acccagccgc tctcatcact actggggctg gaagagaccg 241 gatgagtttc ggagctgagg gcagagggcc aaagccaggg cttttcatgc cactcggaaa 301 cagatctcgg ccatggtgtc ctactgcatt aggcaagcaa gatgcaaaga aatcctgggg 361 tggaaagaaa tctggtccag gttcgtgccc cctgggggtg gatagtgtgg cactccatgc 421 ctgccgctgc taggcatgct caatcccacc tctgagcttc tccaacccct gcgcgcccac 481 tcgtccaagg gcctgatgaa tatcagccaa gccagcccaa gctgctctcc actctcaact 541 tgaacaacca tttactctag gttttctggt cctctattag tgaaattcaa actacagata 601 aaactagctc gtttcttata gtgatgaccc caccccgacc ccattcttaa ggaagtcaca 661 gcattatc //