LOCUS BG076061 662 bp mRNA linear EST 12-MAY-2010 DEFINITION H3155B08-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3155B08 3', mRNA sequence. ACCESSION BG076061 VERSION BG076061.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 662) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG076061.1. Other_ESTs: H3155B08-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3155 row: B column: 08 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..662 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3155B08-3" /db_xref="taxon:10090" /clone="H3155B08" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 155 a 148 c 148 g 211 t ORIGIN 1 attcttcttc gcctcggttt gctcttctgt ggtgccattt gtttttgcag atggaacttt 61 agtgatcggc atctctgcat gtggcttggc cgtgtggctg ggtggctgct gctgatcttg 121 ctcactgctg gatgcttcgg agaagatatc ccacacctgc tctagaacgg agtcgctgtg 181 cactttcagg ctgtttttca tccagttctg aaacttcgcc ttttttctgg gaacattatc 241 aaaagcactg atttgctgca aaagttctcg caccttgggg ctgacgttgg gtttctttat 301 taactcatta attttcagaa atctttccca cagtcaatgc aggaaagaca ttcacagttt 361 ctgcagttag acacatgctt ttccacttgt attttcttca cggattcacc acatgcattg 421 catgtaaaaa ataccatttt tggttaaata attagaaatt ttctgatata ggtttgaact 481 cttgcctaac ttagagcttt caacctctgc ggctgacgac tgctgtttct ggtcaagctt 541 gagaggagag ggcaggcttg agtaaacctt gggaatccac gggtctgcag caactatgaa 601 aaacagctcc ccatggcaca gtgtgaacac caactcaaga gaaggctgga atcaggtcat 661 at //