LOCUS BG075867 695 bp mRNA linear EST 12-MAY-2010 DEFINITION H3152G08-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3152G08 3', mRNA sequence. ACCESSION BG075867 VERSION BG075867.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 695) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG075867.1. Other_ESTs: H3152G08-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3152 row: G column: 08 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..695 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3152G08-3" /db_xref="taxon:10090" /clone="H3152G08" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 188 a 182 c 132 g 193 t ORIGIN 1 aaccggacca tgatttattg tccccaactt gacagattaa tttggcaatt tgatgcccaa 61 tcatattgaa taacaaaaat atacaagtga catgtaaaat gtctccaatc ctctcattgc 121 ctccctaatg tcactgccct taatgacctc ccaaaccaga ggctacacgt ccctcctccc 181 ctgggtcatc ctgttgtgtc acctttccag atattccaaa aaccttaccc ccctgggaaa 241 gccagtgaaa gaatgaggct ctgtccaggg gtgagcacag gagattccta gcaggcctca 301 agactaggat tcacaagccc taagtcctag acctgttttt acaagggtct cactttgcaa 361 agtaagatga ttcagactca aggacagttt aagctttgcc agaatccaga gagaccatcc 421 tttggatttt gtgtctaact tggctttcct catcgcctgc cagagacttc agcagtcact 481 cgaccacact ttaattgtct tgtcattttc tagtgctgct gatgcgatga tgttttttgt 541 agggtgacag gctgctgaga tcaccacatc tgtgtgacct tgcaacctct gtacaatctc 601 tttagtctga agattccaga tgtaaaccat gttgtcctct gaaccagaca caacccactt 661 tctaccagta accgagaaac tggcaaatag gcagt //