LOCUS BG075598 584 bp mRNA linear EST 12-MAY-2010 DEFINITION H3149B10-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3149B10 3', mRNA sequence. ACCESSION BG075598 VERSION BG075598.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 584) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG075598.1. Other_ESTs: H3149B10-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3149 row: B column: 10 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..584 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3149B10-3" /db_xref="taxon:10090" /clone="H3149B10" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 111 a 159 c 163 g 151 t ORIGIN 1 cttttttcga catcatcggt acactttatt cctcagggca ttacacgcta acttttcctg 61 gagcgggtca gtgcatcgag ttctgtttct gtggaagatg tgcacctttc caaacggcgt 121 ggctggctgt ctcaccctcc ggtagggcct ctgtgcacag gaagcggttc ctccagcaca 181 aagccagcct ctggggcctg gacacaagag attgctctgt ctagaagtgc ctgttggacg 241 cccctcttcg gtcttggtgg ccagcatccc tccctggctg cttggagcct cggtgcccgg 301 cctggcatgc atatgctgta gttaagggta aagtgacagg gtcattcaca ggcgtgtcca 361 caacagagat gctcatggcc tcacacggac tcctcagcag acagcagaag tgggttgatg 421 tactcaaagc cttcaaattc ggactggtcg atcctcttta tgacgtcctc atcatctggg 481 gtcagctgca caggctcgct ggtgaactgc gtgtcaaagt tgtccaggcc atagtcatct 541 gtgatctggg gctggaaggg aggcagggtc tgcttctttt ccag //