LOCUS BG075387 684 bp mRNA linear EST 12-MAY-2010 DEFINITION H3146F04-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3146F04 3', mRNA sequence. ACCESSION BG075387 VERSION BG075387.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 684) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG075387.1. Other_ESTs: H3146F04-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3146 row: F column: 04 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..684 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3146F04-3" /db_xref="taxon:10090" /clone="H3146F04" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 174 a 186 c 150 g 174 t ORIGIN 1 ccaaaacaga cttagccctt gacatttatt tcatggttcc tggagttaaa ggtgcaccct 61 aactcattgc ttagcagtag gttcccaaat atttatttca tcatgatctg agtacaggag 121 tactgtcctg acatacccct agtgcagaac agaaaccaga aaggggcaga tctgtccact 181 gccctggcct ccccagactt tacctgttta atcaggaaaa gcttcattca agtacccaga 241 ccctaaggac aagaatcacc aaaatgccca tagtgtggat ccaagccaca gctttgaccg 301 cttgtctttc cagaatgagg aaggcagtgt gcaggtcttc tttgggcatg gctcctcaga 361 gcagcccttc tagctctgct aagaactctg ttcttgtctc agaaccagca gcgtggctga 421 cagctcagtc tgtttagaag agctcagccc tttggtctac ttcatgattc acttgaactg 481 agcagcccat aggagcatca agactgattc cagctgcaca cagactccag gcgaggctct 541 ccagcttggg agctagtcct gaagcaggtt tcgagagatc agcatcctca tcacttcatt 601 gctaccttct aggatctggt ggaccctgga gtcccgcata tactgttgaa cagcatagtc 661 cttcagatag ccgtagcccc catg //