LOCUS BG075349 828 bp mRNA linear EST 12-MAY-2010
DEFINITION H3146B11-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3146B11 3', mRNA sequence.
ACCESSION BG075349
VERSION BG075349.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 828)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG075349.1.
Other_ESTs: H3146B11-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3146 row: B column: 11
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..828
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3146B11-3"
/db_xref="taxon:10090"
/clone="H3146B11"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 254 a 174 c 195 g 205 t
ORIGIN
1 agtaaagttc ttatttctca acaaaattaa gcaaacaagg ttaggttgac ttggtcatgt
61 ttatacgcag gcacacatct ggaatgggct tggcacagca tggagggcag gcaaatcgtg
121 attgaaggct acgtgctttg agaccttggt gaattacctc acagttctga aactctgaag
181 tattacagtg gcagctatac tgtgaacata cctgccaaac ttgccgtatg ctcatgtatg
241 tgggaacact gttagatttg gacaccggga aggatgtttg tatgaaatga agtgtctgga
301 gtctaacagt acaaattaca aagatgttat gtatcaaagt gtcctaatga agtccactat
361 gtagtactct aactaactta aaaatgcaat ggaaagagcc aggaaaacaa gaagctgcag
421 gaaagcacag ccaagccagc atcatatgca ggtcatgggc acagtgatct gcgcacgctg
481 ctcactcttt cctcatgaca gaatgtacga gaacctgtca aagcgagtgg agtgctgctc
541 cagcaggtgg atctgcatgg tctccaccag ccgagtcact cagggaggct tgggtcagca
601 tgggttatgc tggacgttat gcgttttagc aagcacaaaa ccctaagaca ctgtcacctt
661 tttaaaatca aaacacacat ttgcctccga atagagcctt gctactcaat cccaagaggt
721 aacttgggct cctctaaggt agctaaagga cagaaaggct atgtatgaag agtttacctg
781 ggaaaaaact cacaaaacgt ctatcatcct gctcctgaat attaggga
//