LOCUS BG075288 729 bp mRNA linear EST 12-MAY-2010 DEFINITION H3145E04-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3145E04 3', mRNA sequence. ACCESSION BG075288 VERSION BG075288.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 729) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG075288.1. Other_ESTs: H3145E04-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3145 row: E column: 04 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..729 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3145E04-3" /db_xref="taxon:10090" /clone="H3145E04" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 155 a 221 c 218 g 135 t ORIGIN 1 acctgcgcag gcgctcagcg ggcagaggcg cacgtggcac acagtggcag cccgttagtg 61 cctctcccac aggagaagtg gtacattgtg ttctcttgcc aggcttaccc agtgcggggc 121 ctgggccact ttgcagtagc agatgctgcc atgccctcct gagtgacaca tgacacatct 181 gtgctcaggg caggaccctg tgccactctg gatccatggg gaagaaggtc cacaacccaa 241 gccatgctgt ggctctggga gagccccgga aggcaagcag gagtggggct gggtggtggg 301 cattagagaa gccaagcagc cccacgccct gcctctgtaa acagaccctg gtgggtccct 361 tctcggcccc aggcacatcc ctgagaaaca ccggggactg gttgcagctc tttctgggag 421 gccaggtgat ccaccaagcc agctgcaact ttattctgtc ccctccctcc ctgcagagca 481 gcttctgctg ggttgaggcc cagcacctgc agcgcctgac ccaagggcct gaacaccacc 541 aaggtcttct gcacaaaaaa caatgtagag tgggccagac cacatggggg cagacataat 601 ggctggggga cataggtggc agggtggtcc tgggaatgtg ggtgcataga cagcagcctc 661 ccagccactg gcaggccacc tcttcccaga caatcaattg ccctaggcat gtttagagct 721 cctcatagt //