LOCUS BG075150 600 bp mRNA linear EST 12-MAY-2010 DEFINITION H3143H03-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3143H03 3', mRNA sequence. ACCESSION BG075150 VERSION BG075150.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 600) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG075150.1. Other_ESTs: H3143H03-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3143 row: H column: 03 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..600 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3143H03-3" /db_xref="taxon:10090" /clone="H3143H03" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 139 a 131 c 131 g 199 t ORIGIN 1 cataacagac gcgtttataa tccaaatgtt gtagatttac aatacctatt tatttgtacc 61 aatttatttg taaatcttgt gattgttttt aaaaggtttt gtttatttct attcttctat 121 ttagataaat gattttctgt tcaccctttt agcagctgtg tgttccctct ttcctctgtg 181 tgacaccaac cattttgttt tcttcagccg tggtcccgta ccggtttaca tggctttcct 241 tgtgggaaag gcagaccggt tcacatcagg gcgtctcctc tggacagaca ctgtaggttg 301 agtacctgca tgttacagcc tggtacacag ctggtttggg ggatgctctt ctgaggaatg 361 aggtgcgccc ccaccagctc ttccctagga aacgtacaag tatccatctg acttgcctag 421 tgacacacta ggttttcctt ggcagtaaca aaggcttctt cgtacaggcg tctcagttgg 481 ctcttgaaga taaaaatggc tatttagctc ctctaaacca gtgaggaaag tcagttcatg 541 ggtgagtcac cctcaagtga tacaaggcca caggtgtgga ggcatattag atgttcttcg //