LOCUS BG075138 718 bp mRNA linear EST 12-MAY-2010 DEFINITION H3143G02-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3143G02 3', mRNA sequence. ACCESSION BG075138 VERSION BG075138.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 718) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG075138.1. Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3143 row: G column: 02 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..718 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3143G02-3" /db_xref="taxon:10090" /clone="H3143G02" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 159 a 189 c 168 g 202 t ORIGIN 1 gctctcaagt ctctttattg gtgttttaga aaaaaggcct ctaccatcaa ggtgaaaact 61 ggacaggcag aagcagcact ctgaggctca ataccaataa actttcttga atcttcgagt 121 ctcctggatc cccatggcct ccatcacatc ttcttctaat gtctttagtc ggggcacata 181 agttctttca agagcatctt ccactgatgt gtccttgggg ccatcagtcc atgtttcagg 241 gacaatgcca tctgctctag gaaattcagg tttggggata attctgccag atcttgtaga 301 gactcgaaca cgttctcctg cctcagtaaa tctccactgg atctcagtgg gtttcctgtc 361 cacaggatcc actagcttga cctggtgatg aagcaagggg gcttcactag cgatcatggt 421 ccctcggtgg tccttggttc tgccaatgta tcggtaatgc gtgttcaacc cctccaggac 481 aacccagttt cgctgccgaa cgacttggac tactttgcct tgcttcccag catccttgcc 541 ttctaagatt tctaccatgt ctccacagaa taggtgccag tcttcatcag agatgggctc 601 taccactact gggcaccgct gctccatgga ggattcttcc ttttgtctgc tatggaacct 661 ggacggctca ttccatagcg gtagaagggg gaggaagtgg ctttggatgc aaggccag //