LOCUS BG074943 556 bp mRNA linear EST 26-JAN-2001 DEFINITION H3141C05-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3141C05 3', mRNA sequence. ACCESSION BG074943 VERSION BG074943.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 556) AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A., Tanaka,T.S., Carter,M.G. and Ko,M.S.H. TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set JOURNAL Unpublished COMMENT Other_ESTs: H3141C05-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3141 row: C column: 05 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..556 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3141C05-3" /db_xref="taxon:10090" /clone="H3141C05" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 163 a 102 c 155 g 136 t ORIGIN 1 caagaaatct taatttcttt attgtttgac ttttttgact caacattttt ttaaaacttt 61 ttgttttttt ctgaaacgtt cttatgagcc ttttgtttta ttctcgttaa atgcactttt 121 cccaaaattg gtttggcata tcgaaaagga gaccaaggag gggagggagt agggtgtggg 181 agcagggagg aggcccaaat ggactaagaa attgagaatg agaggaacac agagacagca 241 aaaaaggaca caacggaaag ttgaatagct aggaaaactc aaaagaaaaa gacctagaga 301 aaaagaatga gcaagattca ggagcaaatg aattcaggag cccaaggaag ggaggaggag 361 accaagaccc ttctctgtac cgtccccgct ggggtggggg cgtcaaggca ccaggtctgg 421 ttagggtagg ggacacctgg gctctctggg tggcttacgc tggattgtag tggatccagc 481 cccacttggg gacctgccat gtagctctga ggcctagaag caagttgctc cctgtgctag 541 tgattccagc taggca //