LOCUS BG074893 550 bp mRNA linear EST 12-MAY-2010 DEFINITION H3140F05-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3140F05 3', mRNA sequence. ACCESSION BG074893 VERSION BG074893.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 550) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG074893.1. Other_ESTs: H3140F05-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3140 row: F column: 05 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..550 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3140F05-3" /db_xref="taxon:10090" /clone="H3140F05" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 112 a 147 c 155 g 136 t ORIGIN 1 gctacacggt gggcagattt atttggggaa cattagggat gaatatatgc cttccgcagg 61 caaaatctgc caaagtcacc tagaagctgc aggttcacat gacaaggatg ctgagcaggg 121 acccagggct ggttaggagc ttccaccaag tgaccacacc aggctgggcc actctgggct 181 tgggagtctg aagtcccatt tcccaggagc tcttgagtgc tgttctgtgg aatggagtgt 241 gacatctagg gtcattcttt ggctgtttcc aaggcttagc gtgtggaaga cacaggaaag 301 gccccgcaaa ggcagccagc cttcacttta tggaaagtgc ctctgttgtc ccttcccagg 361 ctctgccaca ttcttggagt caccttctgg atattccttg tgaagcctct gtcgcaggac 421 ccgcccttct gcttgccggc gttgcttaac acactgtcca tggttgggta tgccctgggc 481 ttcgtcccct ggctgcagga tatggcactc atagcctgag ggacagagga gtggctctgc 541 cccatcctca //