LOCUS BG074388 647 bp mRNA linear EST 26-JAN-2001 DEFINITION H3134E06-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3134E06 3', mRNA sequence. ACCESSION BG074388 VERSION BG074388.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 647) AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A., Tanaka,T.S., Carter,M.G. and Ko,M.S.H. TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set JOURNAL Unpublished COMMENT Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3134 row: E column: 06 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..647 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3134E06-3" /db_xref="taxon:10090" /clone="H3134E06" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 155 a 155 c 154 g 183 t ORIGIN 1 accatttata gagcttgtat taaagccaca agtttagctt tctgggctga agtcccttta 61 gggaggctgc tggcctagat cacttgcttt ctgtccatca ctgcactgct gtccccgcct 121 ttcgcttacc tttaaccttc aggctactgc catccgtgta ccagctcgaa ctctcaggcc 181 aaggctgatc tgatccttca gatcatttcg agtatgagtt ccttctgcca gaatgtcagc 241 acaatgatga gtaggtgaag tctcaggcag tagtgagatt gaggatagca gggggagcga 301 aggtcactcg ttcagtcaac aaaaagctct gataatgtgt ccatcggtca gggggacggt 361 tggataatgc tttcaagagc gttagtcagc cattggtcat gggggctgaa ccacactttc 421 taggacatgt gccaagtttt gtctcagagt caatttatct gcgtccttga ccagaagcca 481 ctatgtgcag acaagcaggc catcttacag ccaccaggtc taatttctta caagtatgcc 541 acaggtcttt ttcagggtcc caatctctga gtaagaactt ttttggcaat acctttgttc 601 tcagtcacat ctaggagtcc catagctggg gataacatta gggcagt //