LOCUS BG074350 646 bp mRNA linear EST 12-MAY-2010 DEFINITION H3134A12-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3134A12 3', mRNA sequence. ACCESSION BG074350 VERSION BG074350.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 646) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG074350.1. Other_ESTs: H3134A12-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3134 row: A column: 12 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..646 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3134A12-3" /db_xref="taxon:10090" /clone="H3134A12" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 156 a 178 c 143 g 169 t ORIGIN 1 ccatttagat tacggctgac tctgagctcc tgtctcactg ctggcaggag actttgctgt 61 tactgggact gtctccttag ggcaggtcac ggccctcagt gcacacaacc ctcgaaactg 121 ttttctgcaa tggattccgc tgagctggcc agggtcttgt cttccataaa ccaaaaggcc 181 cctgacactc agacagaaaa tctggtgaag cagaccatgg cgcctagggg tctccctgtc 241 tctcaaggct tgccatcacc atccaggtcc tacagtggaa cctgcgcata cccaaatctt 301 cccaggtcca catagctctt tcttctgtgc tcctcccaat gaaccaacca gcatctcccc 361 ttccatcttt ttctcctttc tgccttcaac agcatcgttc tcattgtctc cttcttctag 421 gctcacatgc tagtctttct ttagagttta ggctataaaa aaaagataaa aggcacatgg 481 gacttccaat agcctctgcc ttctatttcc aaaaatccct acagtagaga aacgctaagg 541 gagctaccag ttacttagca tggtaagaaa gtggggatga gaaagtcagg agggaagggc 601 accctggaat ctaggtgtat gggtcggagc tgatcctatg ggagga //