LOCUS BG074188 721 bp mRNA linear EST 18-DEC-2003
DEFINITION H3131H05-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3131H05 3', mRNA sequence.
ACCESSION BG074188
VERSION BG074188.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 721)
AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A.,
Tanaka,T.S., Carter,M.G. and Ko,M.S.H.
TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set
JOURNAL Unpublished
COMMENT On Jan 26, 2001 this sequence version replaced BG074188.1.
Other_ESTs: H3131H05-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3131 row: H column: 05
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..721
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3131H05-3"
/db_xref="taxon:10090"
/clone="H3131H05"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 206 a 164 c 158 g 193 t
ORIGIN
1 ggtgtgtgtc aatgatgaga aaaaacaaac attttggttt aatttggaaa gcagagaaac
61 aactgaaggc tgtggtagcc cagccaagaa tagcccagca tgacaaagcc cttgcttctt
121 acagaaaagg agggagacct ttcaatatgc ctgctggcct tctgctgtca atgggagact
181 gtcagtcata ttaaatcata acattccaat actgcttcta ctgtaagcca gccttgcagg
241 cgagagctca gtgcttctga tttacaaacc gtcattccaa ggtggtaatg aagagcccac
301 tgaaatgtca ggagggtcac tcagacatgt ctgtgatcga gtccatttgg aaaacatgaa
361 gctcacagac actctgttag ggctctgtat gcgttttctc ctctcctatt tgacatttag
421 cttggtcatg aatttatttc gcactgtgaa gtatggttcc cccaacttct ctgacaagaa
481 aaccatgtac ctgaaaaaac atttttagca tacgtgttct cttgtgttga ggaccgtgtt
541 gaggaccgct agattcctct attcctgcaa gatgcaccca actagcccaa agtcctaaat
601 gagaatgatg taaccagagg ctgttcaggg ttcatagttc ataccgccca cccccaccct
661 atctgaccag cataaagagc tgaatcagga gtgctggtgt acaaacaaag gctattttgg
721 a
//