LOCUS BG074149 662 bp mRNA linear EST 12-MAY-2010 DEFINITION H3131E12-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3131E12 3', mRNA sequence. ACCESSION BG074149 VERSION BG074149.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 662) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG074149.1. Other_ESTs: H3131E12-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3131 row: E column: 12 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..662 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3131E12-3" /db_xref="taxon:10090" /clone="H3131E12" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 206 a 164 c 137 g 155 t ORIGIN 1 aatttttcaa tcttcattta tcagtcatga tatgtacaag acaaacaaaa aacaaaaaca 61 aaaacaaaca aaaaaacaaa aaaaaacata tacacaaact gctttaaagt acttcattaa 121 aacaaaggtg aaccaaacca ttcagaatga tgtaaaatca tgaacaaaaa taagaactaa 181 cataaattat caatacctac acatcgaagt agtcagttag caaaacaaag ctagcacctg 241 gtcacccaga aaggccaagg gacccctccc caaccgttac ttcactccat ctctctctgc 301 ctcaagcttc cctcctttca cactggggta ccaggcagga ggctcctgga cgtggctaac 361 cgtgccgcag ctcccaagac agctgtggct gtacttcgag ggatgtagag cttctgagaa 421 atctggtgct ggggggacaa aatgaaagag agctggtcta tggctccctg actcttgtcc 481 ttggccgtgg cttcagcacg gctattagaa acatcttctt tctttaggct ccctggagag 541 ctttccacag agccagagtg gttttctgga gagtcaccca ctgcaggggt tgaactgagt 601 gcttttgagg ctgcccgtcc ggcatgagtc tgaatgctgg caggttcaga ctggctcctc 661 tc //