LOCUS BG074039 635 bp mRNA linear EST 12-MAY-2010 DEFINITION H3130B05-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3130B05 3', mRNA sequence. ACCESSION BG074039 VERSION BG074039.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 635) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG074039.1. Other_ESTs: H3130B05-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3130 row: B column: 05 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..635 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3130B05-3" /db_xref="taxon:10090" /clone="H3130B05" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 168 a 146 c 157 g 164 t ORIGIN 1 aaacccaaga gaattttatt taagtagtat gtgacaaata tctaagaaaa ctatttcttt 61 acactgctct tgtactcctt tcactgggta cgttcttata actttgtttg ttcttagctc 121 tgggccctgc agaaacacca cggtggacgg aggcatagtt ttgttctgcc ctcaacaggt 181 cagaagttgt tgatcttttg tagcagggtt tggcgccgct cctcagggct aatgttcttg 241 gcgatagact ttaagaagcg ctgctcgtct accttctgga gcaaggagct gacagaaggg 301 ttgcttttgc ttagagtctc gtagcaggta aggatttcca aggccagcac gtagaggggt 361 tcacagacct cctgagggag catggccatg atatgcagaa catgacagaa catctgggta 421 tagacaaaca gggtttcctg agtaagatcc ccttcttgtc cttgggccca gggacccatc 481 gactgcatca acctgactgc atccaggaca ctggtcagac tgttacagag gagtttgacc 541 acatggctcc aaccgtccaa aggaagccag ttacaacagc tctggctgca gagaaactgg 601 aacactctca aagaaagaac agagagctga agttc //