LOCUS BG073993 564 bp mRNA linear EST 12-MAY-2010 DEFINITION H3129F04-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3129F04 3', mRNA sequence. ACCESSION BG073993 VERSION BG073993.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 564) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG073993.1. Other_ESTs: H3129F04-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3129 row: F column: 04 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..564 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3129F04-3" /db_xref="taxon:10090" /clone="H3129F04" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 152 a 124 c 103 g 185 t ORIGIN 1 ctgtttcaaa cattttattg caagatgctt tagttaatta caagtttaaa ttataacttg 61 gtaatttcag ttgggcagca agttgctgca agttgcttat tcaaattatg atcagataac 121 tccgcataca gagcaaggat cagaaatacc ttactggcta atagggttaa gccactaagt 181 ggaccttgat cattttattt caatagatag caatacagaa agagttcttc acatatacta 241 gttcccattt aactctctct ggctatcaca actgagaaag acatattcac ataattggct 301 gagcttgagc cttctttgca agcagcttta gatcttcaat gcacttggca atggtctcct 361 tttcctgctg tacagaaatg ctcttcacca catgcttttc tacccagtct atcatgtgtt 421 cttcctcctt gcgacgcatc atgttctgta cagagatgtg gtagtccagg cgattcttta 481 cctccttata tgctttatgt agccgttccc ggtaagtgac ctccaaggcc agggcaatgt 541 tattcctctg cacatcgaag aggt //