LOCUS BG073970 553 bp mRNA linear EST 12-MAY-2010 DEFINITION H3129C12-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3129C12 3', mRNA sequence. ACCESSION BG073970 VERSION BG073970.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 553) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG073970.1. Other_ESTs: H3129C12-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3129 row: C column: 12 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..553 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3129C12-3" /db_xref="taxon:10090" /clone="H3129C12" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 182 a 106 c 118 g 147 t ORIGIN 1 catacaatgt tattcacacg tttcattttc taatttatac ataatataca aagtgaagat 61 aacatttcat ttgagccaat aagagccagg acttagacaa actggtccag aaacaatcta 121 catcaaaaag agactactca agaattaaag cctaagtgga ttctgcccaa tcagatgtct 181 ttattctgta ttgtcagatt tttctaaagt tccatgccaa catactagaa ggtggaatca 241 gggtgggcac caaaactggc agatcataaa ttttctacac gaacaggttt tttctagaat 301 atgcctaaaa gtatcaggag gactgggttg tcagcactgg gaagtatgaa tcaggactgc 361 tagaggagga ttcagttcca aataactttc tctaggactt gccacttttc tgagccgagt 421 gacagctgcc ggggcatcca aagggaaggg tgaggacaaa ccagaatggg ctgcatttct 481 gcaggaagag ctgttactaa tctctaacac caacaacttt gatccgaaac tatgggctga 541 tgagaggaag tta //