LOCUS BG073906 582 bp mRNA linear EST 12-MAY-2010 DEFINITION H3128E11-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3128E11 3', mRNA sequence. ACCESSION BG073906 VERSION BG073906.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 582) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG073906.1. Other_ESTs: H3128E11-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3128 row: E column: 11 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..582 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3128E11-3" /db_xref="taxon:10090" /clone="H3128E11" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 143 a 154 c 168 g 117 t ORIGIN 1 acatctaaag catcgcttta ttgtctgtca gtcacagggg tgccagcccc tgcctgccca 61 cccctgtctt ctcacaggct ctctgggctt atgtgtgtcc tgagaactga cagttgtctt 121 aactagccat ggtgaggaga caggggagag ctgctggaca aagtgggagc agtgcggaga 181 catttgtcca tccttcattc tgcaaactgg tccaagaact taaggtaggc ctgacgctga 241 gatgcagctg ctggaatgaa gtgaccaccg gagtgggtga gggtgacggc tccaaggaat 301 cggctagcca attgcatact ctcctgagaa gggatgactc gatcagtatc cccaaaaaca 361 tgaagtgaag gcagtgacat ggggctctgc aagataggtt ccttaaggcc tcggggacag 421 aaccccgacg caagtataat aaatcttggc aaggggaagc gaggatcgcc tgcctggccc 481 agggcacaca cataagctgc tagtgcagcc ccctggctga agccaagaag tccatcaaac 541 ggccccagcg tgtcaagggc ccgtgccacc gtttccaggg cc //