LOCUS BG073788 575 bp mRNA linear EST 12-MAY-2010 DEFINITION H3127B02-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3127B02 3', mRNA sequence. ACCESSION BG073788 VERSION BG073788.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 575) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG073788.1. Other_ESTs: H3127B02-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3127 row: B column: 02 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..575 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3127B02-3" /db_xref="taxon:10090" /clone="H3127B02" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 144 a 139 c 82 g 210 t ORIGIN 1 cataaagact tcatttttca tgtaatcaat aagtggaata caggcattag ttgtgattgc 61 aagcatatca gtgagaacta atctctgact ccccacaatc ccccgcgcct tggctccctt 121 caaccttcaa gatggagctt agaatctgct tccttcattg ttatgcacaa agccacattg 181 gcacctgtat ctgtagtatt gttgtatcag tggccttggc tcaccagcgc cacagctggt 241 ctcacactct tgcacactcc tgagtgttca tggcacttcg atcccttagt ccctaaattg 301 caaggttttg cactctcaga gttgaataaa atcagtgcct agaatgcact gccacccctc 361 cacacattta attaaagcaa cataattatt ttgaaaaata ttatcatgtc tacctccaca 421 tagataagtg tgctcaatag aaaatctctg gatatgttat gagtttaatt tctcttcttt 481 ctttctttct ttctttcttt ctttctttct ttcttccttt cttttttttt tttttttgaa 541 aattgacaat tattctctat tccagttttc cacca //