LOCUS BG073539 719 bp mRNA linear EST 12-MAY-2010 DEFINITION H3123H01-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3123H01 3', mRNA sequence. ACCESSION BG073539 VERSION BG073539.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 719) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG073539.1. Other_ESTs: H3123H01-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3123 row: H column: 01 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..719 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3123H01-3" /db_xref="taxon:10090" /clone="H3123H01" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 166 a 177 c 185 g 191 t ORIGIN 1 ggaagtttga gaatttatta ggcaccgagg gtgagtggag cagagttaca aagtggctgt 61 tagctttcct tcaaattgaa aaggagctgg tgtttctcct gccttggccc tttaaggctg 121 cattcgaatg gccccatcca gccggatgac ctctccattc aagaatgggt tctcgattat 181 ggtctgtacc agatgagcat attcagcggg gtcacctagt cggctgggga agggtacctg 241 gctggccaag aagtttcgca ctttctctgg aagggtggta agcagtgggg tggcaaacaa 301 acctggagca attgttacca cacggatgcc tgtaggagcc agatctcgag caatgggcag 361 tgtcatgccc actatgcccc ctttggatgc agagtatgca gcttgtccaa cctggccctc 421 aaaggcagcc acactggcag tgttaatgat aactccacgt tggcctccct ggtctggttc 481 attctggccc atctccccag caaccaggcg gatcacattg aaagtgccta taagattcac 541 attgataacc cgctggaagt cctccaaagt atggatcttg ttcttctttt ggtggtatgt 601 cttaatggcc actgcaatac ctgcacagtt gacagccaca tctatacggc caaacttttc 661 ttttgctaga gtcaaagctg cttgtatctc cttctcagag gtcacatttg ctggggcaa //