LOCUS BG073527 721 bp mRNA linear EST 18-DEC-2003
DEFINITION H3123F08-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3123F08 3', mRNA sequence.
ACCESSION BG073527
VERSION BG073527.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 721)
AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A.,
Tanaka,T.S., Carter,M.G. and Ko,M.S.H.
TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set
JOURNAL Unpublished
COMMENT On Jan 26, 2001 this sequence version replaced BG073527.1.
Other_ESTs: H3123F08-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3123 row: F column: 08
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..721
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3123F08-3"
/db_xref="taxon:10090"
/clone="H3123F08"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 206 a 143 c 169 g 203 t
ORIGIN
1 gtataaaaaa gatttattga aatttatcaa tgacaaacag acataaaact caaagtttgg
61 ctcttctcaa gggcgggaga aaaatgagtg gcagttgttc tgtaccaatg agacgcaggg
121 taggagggac acagcacgtc acttctaaag cgatctggaa gagggatgca gaaggcacac
181 agcattccct aggagaaacc tgcggccatt tcagagtccc accgggtaag aaaatacgag
241 tttgcattcc tttcctgtgc tccaatgtat gtgagaagga aaacaagcag aacaaaaccc
301 cagaggactc ttggagggag ctccagagcg tagatttgta aaagggttct aagctggagc
361 gcctgagatg cgtcctcctg tcatttctgc aaaaccagtt gctctaatat tttacagaac
421 caaatagaac agtttttttt gtttgttttt gtttttaaga agctgaaaca tgaaggttca
481 cttctttccc attttacaag tatctcctaa ccctgccgtt ttttgttttt ttcttgttgt
541 tgttattttg attttgtctt tttggctctc gggatcatag ccccagagta ataagagttc
601 tctttgcaaa aagcaaagcc cactgccatc tgtggaggga gggaaaaggg atgcgagatg
661 gggactgtcc ccaaaccagt gccaaaatac gccttttggt tgctatttac aaaccggggt
721 g
//