LOCUS BG073502 768 bp mRNA linear EST 12-MAY-2010
DEFINITION H3123D02-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3123D02 3', mRNA sequence.
ACCESSION BG073502
VERSION BG073502.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 768)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG073502.1.
Other_ESTs: H3123D02-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3123 row: D column: 02
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..768
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3123D02-3"
/db_xref="taxon:10090"
/clone="H3123D02"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 282 a 161 c 143 g 182 t
ORIGIN
1 actttccact ttacaaactt tattaaataa tgggtattac taagtttcat tgtcttatat
61 acagtattta aagtatgttt tccaagatgc acaactttcc ccccccccat gaagacaaaa
121 agtaggtgaa aagtgaactt atataaaaaa gaaaaattgg acccttcagt acaggaaccc
181 agtaagaaca tttaagtaca taaaacgttc ccagttatgt acatccccac ccccaccttc
241 agaagaaact attttaaaca aaagcattga actgataaca ttgagggaac atctaagcac
301 acactcacac acacacacac acaggctctc aaagtctgca gcctggtcca cagacatgat
361 caataaaact acagctttat cctagagcct aaaggaccac ttacataaga cgttttgtac
421 aatgtgacat tcccctttgc aggtgacacc aaaccatgca atgaaacact ggtgtgaaga
481 attactcatt tctgagactc aaacaggaca gtaacatgaa ctgtactggg atgtgggcca
541 tagagactaa aaaggaaaaa aaaatcagga gggaaaagaa gtcgaattgg aggagctagt
601 tgacacttta atttctatcc cttcttaacc aggaaacaaa ttctccagac tccagaaacc
661 ttgataactg agagcaggag ctcatggaga aaagctgtcg aaactgaaga gaacagaagt
721 tcagatgggg ctctagtgga tgtgaaagct tgagaagtgt cgtcagag
//