LOCUS BG073499 704 bp mRNA linear EST 12-MAY-2010 DEFINITION H3123C10-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3123C10 3', mRNA sequence. ACCESSION BG073499 VERSION BG073499.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 704) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG073499.1. Other_ESTs: H3123C10-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3123 row: C column: 10 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..704 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3123C10-3" /db_xref="taxon:10090" /clone="H3123C10" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 138 a 201 c 191 g 174 t ORIGIN 1 aaaaaaatat ttcagcttta tttggggatg aagacagtga caggagtcag ggtggagagg 61 agcttaacca agccactggt caagtctttg ctgcgaaggg caggcggtcg gtccccacag 121 gtggctcttc tgcaaccttg ctttctctcc gagggctggg aagccagaga aaggaaccca 181 ggtatgcagc gcaggctcac acttcggccc ccagttctag taccccggtt tcaattatag 241 tcacgtattt atcatcgatc tgaacgagaa gcactgtctt gtcgatgtgg gaccttgtac 301 ttgggtctct gctgcaaggc acccatcggt gaaccacatg gccttccatg ccctcctgtg 361 gactccagtc tttccagctg ttcctcccag cttttaaccg aattccttca tcaggccagt 421 gcagctcctt cctctttgac acgttgattc cttccagaat ctgactgaga tccacaatct 481 ggactctgta aatcacttcc ccctttctgg catgggaaat gttggcagga cttgaggtgt 541 tgcctggctg ggcctctgtg gcactgccct gaccgccctc tgcccccagg aaggctacga 601 gggtgtgatg cttgcaggcc gggatgctct ggctggagct gctggaggag ggcaggccgt 661 gctgcacaca gcccatgccc ccctggctgg cagcttccat ctgg //