LOCUS BG073456 734 bp mRNA linear EST 12-MAY-2010 DEFINITION H3122G09-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3122G09 3', mRNA sequence. ACCESSION BG073456 VERSION BG073456.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 734) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG073456.1. Other_ESTs: H3122G09-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3122 row: G column: 09 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..734 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3122G09-3" /db_xref="taxon:10090" /clone="H3122G09" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 255 a 139 c 143 g 197 t ORIGIN 1 actgtgaaat ctgtaataca gaatgattct ttattttgac acatttcaac tgtgaatata 61 acttggtaac taataagaga tgttcacatg aagtaactct gaagccctct taacttctta 121 gtggtccctc aaccattaca aatggaactg atgccaacag atctataggg ctcccagtca 181 cctgctgtcc tgcaaaagga gacaacgccc atccactcca ttaaaacaga aggcatgtag 241 tattgaacag tgcctaaaaa atagagggga ctgagaaaag tacactgttg cctgctaaga 301 aattgtagtt gctttagaat aatagtaaaa ctgagattca ctgtcataac aaagaccttc 361 actgcacgga actaaaaaat agcatgtatg tggtcatgga atgtcacatc agggtttctg 421 tagtattaga ttataggcag ttaacactga tcatatagtg caaactaggt aaagttgcgt 481 ttcactgtgt gaaatgaaga acaggcgcaa atgctcagta ggataccatt aagacagaaa 541 atggcaaaaa ataaatatca aaacttttat cagtgtaaaa aagtaactgg gttattgtat 601 aacctagagt ctggcaaaaa aggcctcaaa aaatttctgt ctttacaagt ctcagaagtt 661 tacaaatgaa gtgcccttcc aggggactag tcacaccaga gactccatgc tctctgcagg 721 gtctgattcc tctg //