LOCUS BG073300 542 bp mRNA linear EST 12-MAY-2010 DEFINITION H3121A05-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3121A05 3', mRNA sequence. ACCESSION BG073300 VERSION BG073300.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 542) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG073300.1. Other_ESTs: H3121A05-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3121 row: A column: 05 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..542 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3121A05-3" /db_xref="taxon:10090" /clone="H3121A05" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 71 a 186 c 141 g 144 t ORIGIN 1 ccatgttcag gtttattgga ggttaagaac tgggcagttc ctttataaaa ggaaaccgga 61 ctgcactgga tcccaggcag cttccgggcc ccaatctggg gcatccatag tccacccact 121 gccggcaggt tcagtttctt ctccttgatg ctgggcgtga gtcctggtgt gtggctctgg 181 gtctcggctt ggccttgcct ctctgggatc taccttgtag cttcagagcc tgccagttgc 241 caccctgacc tcggccttta ttaccaggcc tctgctcctt ccctcgctgc ttcttgccca 301 gagggccccc tgccgtctcc gcttggccgc tgtggggaac aagtcttcgt caggctcctc 361 ccccacagcc tggcggaaat actcagcgtc atcctcttca tcttcaggct gacctgcccc 421 cactgcctct ggcggtgccc ctgggtcctc agcatcactg tcaccgtcgg cccttgcacg 481 ggctcgcttt atgcctgcca ggctcttctt cttgtgggcc tcgcgcagtc ctgcttgcgc 541 tg //