LOCUS BG073270 555 bp mRNA linear EST 12-MAY-2010 DEFINITION H3120F08-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3120F08 3', mRNA sequence. ACCESSION BG073270 VERSION BG073270.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 555) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG073270.1. Other_ESTs: H3120F08-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3120 row: F column: 08 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..555 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3120F08-3" /db_xref="taxon:10090" /clone="H3120F08" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 171 a 111 c 116 g 157 t ORIGIN 1 gaagcttttt tttgagagag agagacacac acacagacag tcagtccact atgctgctct 61 gtctggaact ttctctgtat accagatgat aagagatctg ccggcttctg ccacttgagt 121 gctgggatta aagttgtata aatatacgtg tctttcccct gtttcttatc acatagctta 181 aaatgagctt taggatgcct gagaattaca gcctgggatg ttacaaggcc tctagagctc 241 aagagctcct ttcgcctaag gctgcagggc aaattctaac aaaatccagg ctagttctag 301 cccactatgt agccaatcag gaccctcaac ttttaatctc tatttcccct aagaactaat 361 actacaggca tgtgccatct catctggttt acatttttgg aaataaaagg ctgactactt 421 aaaggcagat ttgaagtaaa aaatttgaag aaagaaaggt aggagattcc acatttgata 481 ggtggagaac agaaaaagat aataagggac attccattgc tagttgagga atctcctttt 541 ctgctaggaa aggaa //