LOCUS BG073188 475 bp mRNA linear EST 12-MAY-2010 DEFINITION H3119G05-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3119G05 3', mRNA sequence. ACCESSION BG073188 VERSION BG073188.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 475) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG073188.1. Other_ESTs: H3119G05-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3119 row: G column: 05 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..475 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3119G05-3" /db_xref="taxon:10090" /clone="H3119G05" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 88 a 133 c 144 g 110 t ORIGIN 1 aagaattgtc catcctttag tccatccttt gttaaaacac tagatcacag ccccgtggca 61 agtgtttgtg tggcagtggc ccagcacgtc tcacttggag ggccgtgtga tgatgcgcgt 121 ggagaagtcg aacaggtccc tgttgatctt gcgcaggcta cgcacctcat cctccagatc 181 aaacacacgt gctggcagca tctggctgct agcaggttct tctccaggta gctggacaac 241 actgcctgca gctgctccag acgctccagg tagctaggct ctttgccctg caggtggagc 301 cctagctgca ggcgcaggcc ctcaccctgg gcttcatctc catgctcagc ccccagcagg 361 tgcttgttga agtggggcag gggcaggctg ggcctgaatt cagggttcag catgctgggt 421 ggggccagaa tgatggctgc attggtcact gggcctttga gggtgactgt gcgca //