LOCUS BG073133 736 bp mRNA linear EST 12-MAY-2010
DEFINITION H3119A01-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3119A01 3', mRNA sequence.
ACCESSION BG073133
VERSION BG073133.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 736)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG073133.1.
Other_ESTs: H3119A01-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3119 row: A column: 01
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..736
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3119A01-3"
/db_xref="taxon:10090"
/clone="H3119A01"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 177 a 164 c 161 g 234 t
ORIGIN
1 ggtttcaagg tactgtgttt ctttatttgt tcttcaatct gccatagttt aatgagtaca
61 tgattctttg acttggcatt cacttctgag ttggttttga aacaatcctt gattattgta
121 ccagctggca tgactactga tctctccatt cctttggagt gactctgcca acaggggaca
181 ggttccagtc tattccaatt tgtgtagctg tatatgtcca tatagaaaca cagaagatgc
241 ctccacctgc taatatagca tttccatatt tgtcatggaa attaggaatc ctcttctgat
301 ggctctgcct tgccaccact tgctgaatgc ttcgaacttg gagatggctt agtgtagtgc
361 gtttttggtc aaggattttg tttttcagaa ctctttgcaa ggatagctgg ggtgaatttg
421 caccaaggca gcagtgtagt caccaccgtt ccatctttgt ctctgagatg ttgcccttgt
481 atcctccttc ttaagatgct tcctgcagaa tggagtgctt caatcttgaa ggaagctttc
541 acacaacaag tcggcagctc aattcccaac aattgttcag tcgcagctgt gaatggaagt
601 ccaaggatct agcagttact cagtctcacg cagcaagcag gcgaaggagc aagagcaaga
661 gctagactcc ctttttccaa tgtccttata ttgtctccag cagaaggtgt agcccagatt
721 aaaggtgtgg tccacc
//