LOCUS BG072763 758 bp mRNA linear EST 12-MAY-2010 DEFINITION H3114F10-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3114F10 3', mRNA sequence. ACCESSION BG072763 VERSION BG072763.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 758) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG072763.1. Other_ESTs: H3114F10-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3114 row: F column: 10 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..758 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3114F10-3" /db_xref="taxon:10090" /clone="H3114F10" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 215 a 190 c 170 g 183 t ORIGIN 1 gaaacagaaa aaatttaatt cagaatatta catgaatata aaagacaata tatatgattg 61 tttctttaca attacatata tatatgcaaa ataataaaaa ataacaaata ggtacaacct 121 tctcctaaga aaccttttgg gggtcgagtg agcaagcaaa tgagcgagcg agcacacaga 181 gcgtgcacag gcacgcttgc tgaaaggcct tgggtgagag cactccttct ccatagcctc 241 tggaccaagc atgtcagggg catgctcaaa agctcttcag catgggctgt ggcctcagga 301 actccagttt ccatggtgac aaagcactca ggttattatt tatcctccat ctcctcactg 361 ccctacctcc acacagcttt cagcactggc tgcctagtaa agccagcttt ttgacaggga 421 gtctgacatc aggctaccag gactaaggtc actcaaaacc ttcatcttag gaagccagat 481 tctctgcaaa ctctgggaca gtcctgctgg tttagatctg gaccctcact tcggtctgaa 541 gactttagag tgtaggagag cagggatgat gctgtctcca gaggaaagga tgtcacagaa 601 aagagacctc agcacacaac ctgccagtca gcatcctgtg ctgcctcatc ccccagggtg 661 tttcctcatc actcctgact gactctggga ggaccttcag agtatagcac atgctgtgtg 721 gagggaccac tgtgggttcc ctcaggcccc atcagctg //