LOCUS BG072755 755 bp mRNA linear EST 12-MAY-2010
DEFINITION H3114E11-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3114E11 3', mRNA sequence.
ACCESSION BG072755
VERSION BG072755.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 755)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG072755.1.
Other_ESTs: H3114E11-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3114 row: E column: 11
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..755
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3114E11-3"
/db_xref="taxon:10090"
/clone="H3114E11"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 190 a 167 c 175 g 223 t
ORIGIN
1 atagaaatga gctgttttat tacaaagagt ttcacaaaat gaaaatatct caaaaaggaa
61 gctaggctat caacctccac ctacagaatt gggggaagac aggcaatata agtttttttg
121 tttgcccaag atgggtggga aggtataaac agaaccaatc taaaaatggc tgctattacc
181 caagcctgaa aaggggtcct aggagaccca gacccctcct aaaaagttta acaggggcat
241 cttagcagag ctcctaagca ggcacttggc agttcaggat ttttctcttc tggttgtttt
301 ctttccacgc aaatgggggg aggggggtgg caggatggac aaggtcgctc tgggaagagc
361 cctcctagca ccactgccct gtgctctggt ccctggggac gttatagtta atatatttta
421 tataaataca cagaggaata gaaaatataa aatctgagaa gtgaggaact tggggagaag
481 atggggctgc agaccttgct caggccaact tgacctcttc tttgacccct ttggcttctg
541 gcttctcctc cttggttttc tcttcttctg tgacttcttt tttctcttca ggatctttct
601 ctttctcatc ctctgtttta actcttttgg cttttttgaa gttagaagag ttcttgcgac
661 caccttctcc ctcctcatct gagtccgaga actcttcctc acaggcaatg cgtttgtcag
721 aggagcagat ggagatgcgt ttgtcagggt cctcc
//